Table 1. Primers, Accession Numbers, and Concentrations used for qRT-PCR.
name | fwd primer (5′-3′) | rev primer (5′-3′) | accession # | conc. |
---|---|---|---|---|
EF1-a | CTACATCAAGAAGATCGGCTACAA | CGACAGGGACAGTTCCAATAC | NM_001104662.1 | 2.5 μM |
CASP3A | CCAAATCCCAGGTCTACTGATG | AGGCAAAGGAGGCAAACTTA | NM_001104670.1 | 5 μM |
BAX | GCTGGTCATAAAGGCTCTCATC | CCAGATTGCTCGAACCGTAAA | NM_131562.2 | 2.5 μM |
BiP | GGAGGATTCTGACCTGAAGAAG | GGTGACAGTAGGCTGGTTATC | NM_001278801.1 | 0.5 μM |
ATF6 | CAAGCCAACTCCAGTCAGTATC | GCCGACTCTCGGTTCTTTATC | NM_001278901.1 | 0.5 μM |
ATF4 | CTTAGAGGTGAAGGTGCCTATG | TGAGGAAGGAGACCTGTTAGA | XM_004066069.1 | 2.5 μM |