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. Author manuscript; available in PMC: 2014 Jun 23.
Published in final edited form as: Nat Nanotechnol. 2013 Nov 3;8(11):873–880. doi: 10.1038/nnano.2013.222

Figure 5. Additional sensor construct with broader in vivo localization possibilities and long term sensing capabilities.

Figure 5

a,b Quenching activity of (AAAT)7-SWNT (red) and Alginate-(AAAT)7-SWNT (green) sensors quantified by percent quenching of original fluorescence using a 785 nm photodiode following exposure to (a) RNS and ROS compounds (analyzed continuously for 10 minutes and once at the 12 hours post addition time point) with error bars representing standard error (b) NO (analyzed continuously for 30 minutes for (AAAT)7-SWNT and just under 45 hours for Alginate-(AAAT)7-SWNT) (dashed vertical line represents a change in the timescale from minutes to hours). (n = 3) c, Image of mouse following implantation of two Alginate-(AAAT)7-SWNT gels on day 0 (immediately after implantation of gel 2) and on day 4 after the fluorescence has returned. (scale bar 4 mm) d, Images of Alginate-(AAAT)7-SWNT gel prior to subcutaneous implantation and at various time points throughout the 300 day test period (scale bar 4mm) and (e) quantification of peak SWNT fluorescence for one of the mice tested displaying long term consistency of SWNT signal. f, Histology images (H&E stained, 10x (day 4 and day 400) and 20x (day 180), scale bars 10 μm) from mice sacrificed at three different time points following the subcutaneous implantation of Alginate-(AAAT)7-SWNT show very little to no inflammation.