Fig. 2.

C5 deficiency protects mice from renal injury induced by cisplatin. Wild-type (WT) and C5 knockout (C5KO) mice were injected with cisplatin (20 mg/kg ip) or saline. Blood samples and kidney tissues were collected 72 h after cisplatin administration. A: serum levels of blood urea nitrogen (BUN) and creatinine were measured. B–F: representative renal tissues stained with periodic acid-Schiff (PAS; B), terminal deoxynucleotidyl transferase-mediated uridine triphosphate nick-end labeling (TUNEL; C), anti-cleaved caspase 3 antibody (D), MPO antibody (E), and anti-nitrotyrosine antibody (F) (magnification ×200). Tissue damage scores were quantified and are shown on the right. The number of TUNEL⁺ cells, cleaved caspase 3⁺ cells, and neutrophils was randomly counted in 10 fields (magnification ×40) per slide and are shown on the right. G: MDA levels were also measured. Values are means ± SE; n = 5 in saline group and n = 12 in cisplatin group (A); n = 5/group (B–G).