Fig. 3.

Administration of purified human C5 restores cisplatin-induced nephrotoxicity in C5KO mice. Purified human complement protein C5 (3 μg/g body wt) or PBS was injected intravenously 10 min before saline or cisplatin administration in C5KO mice. Blood samples and renal tissues were collected 72 h after saline or cisplatin administration. A: serum levels of BUN and creatinine were measured. B: tubular damage scores. C: renal tissues were stained with TUNEL, anti-cleaved caspase 3 antibody, or anti-MPO antibody. The number of positive cells was counted. D: MDA levels were measured. Values are means ± SE; n = 5/group.