Skip to main content
. 2009 Mar;296(3):F496–F504. doi: 10.1152/ajprenal.90443.2008

Fig. 4.

Fig. 4.

Administration of purified human C5a restores cisplatin-induced nephrotoxicity in C5KO mice. Purified human complement protein C5a (3.5 μg/g body wt) or PBS was injected intravenously 10 min before saline or cisplatin administration in C5KO mice. Blood samples and renal tissues were collected 72 h after saline or cisplatin administration. A: serum levels of BUN and creatinine were measured. B: tissue damage scores. C: kidney tissues were stained with TUNEL, anti-cleaved caspase 3 antibody, or anti-MPO antibody. The number of positive cells was counted. D: MDA levels were measured. Values are means ± SE; n = 5 in saline group and n = 6 in cisplatin group (A); n = 5/group (B and C).