Fig. 5.

C5aRKO mice are resistant to cisplatin-induced renal dysfunction. WT and C5aRKO mice were injected with cisplatin (20 mg/kg ip). Blood samples and renal tissues were collected 72 h post- cisplatin administration. A: serum levels of BUN and creatinine. B–F: representative renal tissues stained with PAS (B), TUNEL (C), anti-cleaved caspase 3 antibody (D), anti-MPO antibody (E), and anti-nitrotyrosine antibody (F). Magnification ×200. Tissue damage scores were quantified and shown on the right. The number of TUNEL⁺ cells, cleaved caspase 3⁺ cells, and neutrophils were randomly counted in 10 fields (magnification ×40) per slide and are shown on the right. G: MDA levels were also measured. Values are means ± SE; n = 4 in saline group and n = 5 in cisplatin group (A); n = 5/group (B–G).