Figure 3. Cells co-expressing FOXL2 and AMH in XX Wnt4 mutants resemble activated granulosa cells, not Sertoli cells.

(A) At 18 5 dpc, Sertoli cells express DMRT1 (green) and AMH (red). DMRT1 expression was not detected in the AMH-expressing cells of Wnt4^-/- ovaries (B). (C-D) cytoplasmic BRE-LacZ expression was detected by immunostaining for β-galactosidase (green). At 18.5 dpc, BRE-LacZ localized to somatic cells, including pregranulosa cells, of control ovaries (C) and is expressed in AMH-positive cells in Wnt4 mutants (D and Fig. S3). Whole mount immunostaining was performed for all samples. Scale bars represent 50 μm in main panels and 14 μm in insets.