Figure 5. SAK1 contains an uncharacterized domain present in some bZIP transcription factors.
Schematic of relative positions of SAK1 and bZIP domains. One protein (Cv28) contains a mitochondrial termination factor (mTERF) domain. The letters and numbers in the abbreviated names represent initials of the species and numbers listed in Table 8. Proteins with italicized names contain bZIP domains that were recognized by Pfam but scored below significance.
Figure 5—figure supplement 1. Multiple sequence alignment of SAK1 domains.
The SAK1 domains of 37 chlorophyte proteins were aligned by MUSCLE (phylogeny.fr). Protein identities are as shown in Table 8. Star indicates a relatively conserved residue within the SAK1 domain that was predicted to be a possible phosphorylation site (Figure 5—figure supplement 3).
Figure 5—figure supplement 2. Secondary structure prediction of SAK1 domain.
SAK1 domain modeled against its best-hit nickel cobalt resistance protein cnrr by PHYRE. 44% (coverage) of the SAK1 domain was aligned with 73.6% confidence.
Figure 5—figure supplement 3. Prediction of phosphorylation sites in SAK1.
Prediction of phosphorylation sites by NetPhos 2.0. Orange bar indicates the position of SAK1 domain, star indicates a relatively conserved residue among the 37 members containing the SAK1 domain.