Table 10.
Primers used for qRT-PCR analyses
| v4 ID | v5 ID | Gene name | Forward | Reverse |
|---|---|---|---|---|
| Cre01.g007300.t1.1 | Cre01.g007300.t1.2 | AGCATGTGCGTGTGGAGTAG | CCTTACCATAGGCCTGACCA | |
| au5.g10700_t1a | Cre03.g177600.t1.3 | CTGGACATGTCGGCTATGAA | GCTCATGTCGTACTCCAGCA | |
| au5.g13389_t1* | Cre06.g299700.t1 | SOUL1† | TGCGTATGGGTGTCCACTAA | TGGGGATCTTCTTCATGTCC |
| Cre06.g263550.t1.1 | Cre06.g263550.t1.2 | LCI7 | TTTGGTTGCGTTGCATGTAT | TCAACGCGGTGTCAAACTTA |
| Cre06.g281250.t1.1 | Cre06.g281250.t1.2 | CFA1 | CCTACAACGACAACGACGTG | GGAAGTTCCAGGATGACCAG |
| Cre06.g298750.t1.1 | Cre06.g298750.t1.2 | AOT4 | CCGTGTGCACAGATTCAAAG | CACACAGCGCCTCCTACATA |
| Cre08.g358200.t1.2 | Cre08.g358200.t2.1 | TGTGGCATCAAGGTGTGTTGT | AACCCCACACCCCTCTCTTT | |
| Cre09.g398700.t1.1 | Cre09.g398700.t1.2 | CFA2 | CGACCTGCTGCTCTACTTCC | GTGTAGGCGGTGGTCAAGAT |
| Cre10.g458450.t1.2 | Cre10.g458450.t1.3 | GPX5 | AACCAATCGCCTAACACCTG | CACTTGCTAGCCACGTTCAC |
| Cre12.g503950.t1.1 | Cre12.g503950.t1.2 | GGAGGGAGTACCACGAGACA | GATTGCTGTAAGGCCGGATA | |
| Cre13.g564900.t1.1 | Cre13.g564900.t1.2 | MRP3 | TCATGACGTACATCTCGATTCTCA | AGGGAATGTAGTAGCGCTGAATG |
| au5.g4402_t1* | Cre13.g566800.t1.2 | TGCTTGGAAGACCCACTTTT | GAGCTGGAGTTGCAGTTGTG | |
| Cre13.g566850.t1.1 | Cre13.g566850.t1.2 | SOUL2 | CCCTCCCCTCCTTCAGACTA | CGTACCTGAGGCGCATATTT |
| Cre14.g613950.t1.1 | Cre14.g613950.t2.1 | CGCCCAACCCCATGATC | CCGCAACGTACCGTGATG | |
| Cre16.g683400.t1.1 | Cre16.g683400.t1.2 | CCTGAACAAACACACGATGG | GAACGCCGTCAAATCATCTT | |
| Cre16.g688550.t1.1 | Cre16.g688550.t1.2 | GST1 | AGTGCGGAGGAAGTCGTAAA | GTAAAAGACGTGCGTGCAAA |
| g6364.t1 | CβLP(RCK1) | GAGTCCAACTACGGCTACGC | GGTGTTCAGGTCCCACAGAC | |
| Cre14.g623650.t1.1 | Cre14.g623650.t1 | GACAACGCGGCCTACAAGA | CCGAGCTGGCGGTGTTAA | |
| au5.g2281_t1* | g16723.t1 | MKS1 | GCTTGAGCGCGAGACGAA | CGCTGAAAGCATTGCAGAAG |
| Cre08.g380300.t1.2 | Cre08.g380300.t1.2 | ACCACCAGCAGTACCTGTCC | CGCTCCAATAAAGCCTTCAG | |
| au5.g7871_t1‡ | (Cre17.g741300.t1.2)‡ | SAK1(5'UTR) | CAAGTGCTCATGAGAGGCCTTA | TACGTCATCCAGTTCCACATCC |
| au5.g7871_t1‡ | (Cre17.g741300.t1.2)‡ | SAK1(3'UTR) | TCAAGCGTGTGGGTAAGAGCTA | ACGCTATCTCCGTCCTAATCCA |
| Cre08.g365900.t1.1 | Cre08.g365900.t1.2 | LHCSR1 | CACACAATTCTGCCAACAGC | ATCTGCTTCACGGTTTGGTC |
| Cre04.g220850.t1.1 | Cre04.g220850.t1.2 | TAATGGTATGGATGCGGTCA | ACTGCCAGTTATGGGTCCTG | |
| Cre09.g395750.t1.2 | Cre09.g395750.t1.3 | ACCGTCCGTGAACCTTACTG | CGCAAACACGTCTCAAAGAA |
*
Was originally mapped and identified as augustus version 5 models within Chlamydomonas genome v4.
†
SOUL1 was given the name in v4 but not v5.
‡
Primers were designed against experimentally obtained cDNA (Genbank accession KF985242) and differs from v5. Closest gene model is au5.g7871_t1.