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. Author manuscript; available in PMC: 2014 Jun 23.
Published in final edited form as: Cancer Res. 2006 Aug 15;66(16):8007–8016. doi: 10.1158/0008-5472.CAN-05-4451

Figure 3.

Figure 3

Phosphorylated Akt can reduce nuclear p21 and cause the release of G1-S arrest on TGF-ß. A BPH1 and BPH1CAFTD1 cells were treated with TGF-ß1 for 4 hours. Cytoplasmic and nuclear fractions were extracted. Western blotting results show that, in BPH1 cells, p21 locates in both cytosolic and nuclear fractions. In BPH1CAFTD1 cells, only cytoplasmic p21 is seen. Overexpression of myrAkt1 in BPH1 cells profoundly reduces p21 levels in the nuclear fraction, whereas treatment with wortmannin maintains nuclear p21 levels in BPH1CAFTD1 cells. The p21 distribution pattern was confirmed by immunofluorescence staining. B and C, flow cytometric analysis shows that, after 4 hours of TGF-ß1 treatment, BPH1 cells are arrested at G1-S phase (B), whereas BPH1CAFTD1 cells (C) skipped the cell cycle check.