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Characterization of AcrB mutants. (A) Western blot analysis of the expression levels of AcrB constructs. Expression levels of AcrB mutants were normalized to the expression level of wild-type AcrB. Experiments were performed three times. The average value and standard deviation from three repeats are shown. A representative blot is shown. (B) CD spectra of wild-type AcrB (black) and AcrBL886G/E893G/W895G (gray).
