Table 1. Monomer Stability of Purified AcrB Constructs and Their Activities in the BW25113ΔacrB Strain.
| MIC
(μg/mL) |
||||||
|---|---|---|---|---|---|---|
| protein | Ery | Nov | R6G | TPP | EtBr accumulation rate (s–1) | transition point ([SDS]/[DDM]) |
| no AcrBa | 2.5 | 5 | 5 | 5 | (1.4 ± 0.4) × 10–1 | |
| AcrB | 80 | 160 | 320 | 640 | (8.9 ± 5.0) × 10–3 | 1.6 ± 0.1 |
| AcrBL886G | 80 | 160 | 320 | 640 | (1.1 ± 0.4) × 10–2 | 1.5 ± 0.1 |
| AcrBE893G | 80 | 160 | 320 | 640 | (7.6 ± 3.0) × 10–3 | 1.5 ± 0.1 |
| AcrBW895G | 80 | 160 | 320 | 640 | (1.0 ± 0.5) × 10–2 | 1.5 ± 0.1 |
| AcrBL886G/E893G | 40 | 40 | 320 | 40 | (4.9 ± 0.6) × 10–2 | 1.4 ± 0.1 |
| AcrBE893G/W895G | 40 | 40 | 320 | 40 | (2.3 ± 0.3) × 10–2 | 1.5 ± 0.1 |
| AcrBL886G/E893G/W895G | 20 | 10 | 160 | 20 | (8.3 ± 0.8) × 10–2 | 1.5 ± 0.1 |
| AcrBC493A/C887A | 80 | 160 | 320 | 640 | ||
| AcrBW13C/C493A/C887A | 80 | 160 | 320 | 640 | ||
a
BW25113ΔacrB transformed with the empty vector pQE70 was used as the negative control for the activity assay.