Figure 4.

In vitro complementation of the fully reconstituted DEBS assembly line harboring an AT1-null mutation with the trans-acting DSZS AT in the presence of equimolar malonate and methylmalonate concentrations: (A) Schematic of the overall enzyme system and the expected product, 12-desmethyl-6-dEB; (B) LC-MS analysis of product distribution ratios at varying DSZS AT concentrations. For details regarding the identification of products and extracted ion chromatogram (EIC) quantification, see Experimental Procedures. IS, internal standard, N-Boc glutamic acid benzyl ester (11.6 μM).