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. 2014 May 28;53(23):3796–3806. doi: 10.1021/bi5004316

Figure 6.

Figure 6

LC-MS product profiles for the native DEBS assembly line and the AT1-null DEBS assembly line with and without trans-AT. Methylmalonate was the only extender unit present in the native DEBS and AT1-null DEBS (without trans-AT) controls. Equimolar concentrations of methylmalonate and malonate (DSZS AT) or methylmalonate and ethylmalonate (KirCII) were present in the complementation reactions. All reactions were allowed to proceed for 30 min. For details regarding the identification of products and EIC quantification, see Experimental Procedures. IS, internal standard, N-Boc-glutamic acid benzyl ester (11.6 μM).