TABLE 2.

Rate constants used for simulations

Rate constants were generated for models from simultaneous fits of basal and insulin anti-HA uptake, + LYi transition, and basal to insulin transition data for 3T3-L1 fibroblasts and adipocytes (Figs. 2–4) or from steady state subcellular distribution data in primary adipocytes (7). See supplemental Table 3 for 95% confidence bounds, S.E. values, and data on the goodness of fit.

	ken	ksort	kfuseE		kseq		kfuseG
			Basal	Insulin	Basal	Insulin	Basal	Insulin
Three-step: Δkfuse
3T3-L1 fibroblasts, free fita	0.18	0.053	0.033	0.080
3T3-L1 adipocytes, free fita	0.11	0.350	0.001	0.027
AS160 KD adipocytes, free fita	0.12	0.055	0.006	0.032
Dynamic retention: two exocytic pathways, GSV and ERC
3T3-L1 adipocytes, constrained fita	0.12	0.042	0.025	0.088	0.021	0.059	0.0007	0.025
Primary adipocytes, calculatedb	0.05	0.013	0.018	0.078	0.006	0.143	0.0005	0.05
3T3-L1 adipocytes, alternate fitc	0.11	0.013	0.018	0.078	0.006	0.143	0.0006	0.028
AS160 KD adipocytes, alternate fitc	0.12	0.013	0.018	0.078	0.018	0.030	0.005	0.028

^a Determined from free fits or constrained fits (k_sort and k_fuseE from fibroblasts) of the kinetics data.

^b Calculated from the steady state distributions using Equations 1–3.

^c Determined from constrained fits using k_sort, k_fuseE, and k_seq from primary adipocytes instead of fibroblasts. For AS160 KD, k_seq was estimated from LRP1 surface data (Table 3) as described.