FIGURE 8.

Carbon-13 NMR spectra of metabolic end products excreted by procyclic cell lines incubated with [1-¹³C]glucose. For these ¹³C NMR analyses, the parental EATRO1125.T7T (WT) and uninduced (.ni) and tetracycline-induced (.i, 1 and 2 days) PGKC⁺ mutant cell lines were incubated with 4 mm d-[1-¹³C]glucose in PBS/NaHCO₃ buffer. The NMR spectra of the incubation medium were obtained after addition of 15 μl of dioxane, to quantify ¹³C-enriched excreted end products from glucose metabolism (Table 1). The size of the spectra is normalized on the acetate peaks. The resonances were assigned as follows: A, acetate; L, lactate; M, malate; S, succinate.