TABLE 2.

Excreted acetate from glucose and threonine metabolism by procyclic T. brucei cell lines

The spent PBS medium of trypanosome cell lines incubated in the presence of 4 mm threonine and 4 mm [U-¹³C]glucose was analyzed by ¹H NMR spectrometry to detect and quantify excreted acetate.

Cell lines	na	Acetate production from threonine	Acetate production from glucose	Student's t test (compared with WT)b
		nmol/h/mg protein
WT	38	3155 ± 561	1240 ± 250	/
Δpepck	11	1714 ± 215	1030 ± 136	0.01042
Δppdk	3	3074 ± 316	863 ± 39	0.01370
Δppdk/Δpepck	6	2631 ± 295	836 ± 123	0.00038

^a Number of biological replicates.

^b Statistical significance for the difference in the acetate production levels from glucose between the EATRO1125.T7T (WT) cells and the other cell lines was determined using the Student's t test (bilateral and equal variance parameters). Statistical differences are significant for values <0.05.