TABLE 3.
Intracellular ATP concentration
ATP concentrations (nmol/mg of protein) were determined on trypanosome extracts using the firefly luciferase bioluminescence assay.
| Cell lines | na | Nmol of ATP/mg of protein | Student's t test (compared with WT)b |
|---|---|---|---|
| WT | 18 | 4.10 ± 0.41 | |
| Δppdk | 12 | 3.53 ± 0.38 | 0.00009 |
| Δpepck | 12 | 3.98 ± 0.55 | 0.54389c |
| Δppdk/Δpepck | 13 | 3.55 ± 0.91 | 0.03776 |
| PGKC+.ni | 8 | 3.74 ± 1.32 | 0.33238c |
| PGKC+.i (1 day) | 5 | 2.55 ± 0.69 | 0.00001 |
| PGKC+.i (2 days) | 6 | 1.84 ± 0.30 | 5.56 10−11 |
| Δppdk/Δpepck/PGKC+.ni | 7 | 2.09 ± 0.56 | 2.53 10−9 |
| Δppdk/Δpepck/PGKC+.i (1 day) | 8 | 1.72 ± 0.24 | 2.29 10−13 |
| Δppdk/Δpepck/PGKC+.i (2 days) | 8 | 1.51 ± 0.19 | 1.95 10−14 |
| RNAiPGKB.ni | 8 | 4.07 ± 0.71 | 0.89509c |
| RNAiPGKB.i | 7 | 3.81 ± 0.58 | 0.19717c |
a Number of biological replicates.
b Statistical significance for the difference in the ATP levels between the EATRO1125.T7T (WT) cells and the other cell lines was determined using the student's t test (bilateral and equal variance parameters). Statistical differences are significant for values <0.05.
c Not significant.