TABLE 1.

Substance screening for the ability to stimulate monocultured human primary keratinocytes, HaCaT cells, or trigeminal neurons

Several substances (odorants, 1 mm; histamine, 100 μm; LPA, 200 nm) induced calcium transients in human keratinocytes. Sandalore® and Javanol® evoked repetitive calcium signals in a large number of cells. Experiments were conducted on three different days, with the confluence of keratinocytes or HaCaT cells at 80% and neurons at 50% per dish. NA, not applicable.

Substance	Keratinocytes	HaCaT	Trigeminal neurons
	%	%	%
Citronellol	46	45	33
Javanol®	90	90	5
Lyral	80	80	80
Sandalore®	80	86	4
Helional	NA	27	28
Histamine	93	99	4
LPA	91	96	2
Ringer's	0	0	4