FIGURE 6.

Chromatin immunoprecipitation demonstrates increase in Sp1 binding to the Wnt9A promoter following carrageenan and ARSB silencing and inhibition of the increases when galectin-3 is silenced. A, consensus oligonucleotide sequence for Sp1 in the Wnt9A promoter is highlighted in green. The primers used to amplify this region are in orange type. B, ChIP assay showed significant increases in the measured RT-PCR product following carrageenan or ARSB silencing (p < 0.001). These increases were inhibited when galectin-3 was silenced in the NCM460 cells. C, the agarose gel confirms the increases as measured above by RT-PCR in the Sp1 binding to the Wnt9A promoter oligonucleotide with the targeted Sp1 binding site (as indicated in A) following ARSB silencing or carrageenan exposure, and the inhibition of these increases by galectin-3 silencing. CGN, carrageenan; si, siRNA.