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. 2014 May 6;289(25):17620–17633. doi: 10.1074/jbc.M114.570853

FIGURE 1.

FIGURE 1.

JARID1B expression is disrupted in Jarid1b−/− mouse. A, schematic showing that the JARID1B gene trap cassette was inserted between exons 2 and 3 of the endogenous Jarid1b locus. The location of the insertion site in the protein is depicted below. SA, splice acceptor; pA, polyadenylation sequence; PHD, plant homeodomain; β-geo, β-galactosidase and neomycin phosphotransferase. B, PCR genotyping of Jarid1b+/+, Jarid1b+/−, and Jarid1b−/− mice. The PCR product for the wild-type allele is 611 bp and for the knock-out allele is 350 bp. C, RT-qPCR analysis of Jarid1b using primers corresponding to the indicated exons. Error bars represent S.E. D, Western blot analysis of Jarid1b+/+, Jarid1b+/−, and Jarid1b−/− MEFs using the indicated antibodies. A nonspecific band is observed at 150 kDa (*). E, Western blot analysis of Jarid1b+/+ and Jarid1b−/− MECs using the indicated antibodies.