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. 2014 May 6;289(25):17620–17633. doi: 10.1074/jbc.M114.570853

FIGURE 3.

FIGURE 3.

JARID1B is involved in the development of the mouse mammary gland. A, whole mount preparation of mouse mammary gland from female Jarid1b+/+ and Jarid1b−/− littermates in FVB/N background of the indicated age. Scale bar, 5 mm. The 3 × 3-mm boxed images at increased magnification are also shown at the corners. B, C, and D, quantification of the number of TEBs or ductal ends (in mice older than 8 weeks) (B), side branches (C), and outgrowth length (D) for virgin mice of the indicated age. TEBs, ductal ends, and side branches were counted with ImageJ in comparable areas of 3 × 3-mm squares for at least five pairs of female littermates per age group. Outgrowth lengths were measured from the center of the lymph node to the farthest reaching point of the visible epithelium by ImageJ. For 5–6 weeks, n = 9 for each genotype; for 7–9 weeks, n = 7; for ≥3 months, n = 5. Error bars represent S.E. *, p < 0.05; **, p < 0.01; ***, p < 0.001. E, weight of Jarid1b+/+ and Jarid1b−/− mammary glands normalized to body weights. MG, mammary gland. wks, weeks.