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. 2014 May 6;289(25):17620–17633. doi: 10.1074/jbc.M114.570853

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — JARID1B loss leads to decreased luminal gene signature and enriched stem cell signature without major changes in cell population. A, GSEA of Jarid1b^+/+ and Jarid1b^−/− MECs using gene sets differentially expressed in mature luminal cells (top two panels) and mammary stem cell-enriched populations (bottom two panels). NES, normalized enrichment score; FDR, false discovery rate; DOWN, down-regulated; UP, up-regulated. B, flow cytometry analysis of representative Jarid1b^+/+ and Jarid1b^−/− MECs isolated from females at 7.5 weeks of age. The dot plot shows mature luminal (CD29^loCD24⁺CD61⁻), MaSC-enriched (CD29^hiCD24^loCD61⁺), and luminal progenitor cells (CD29^loCD24⁺CD61⁺). All cells shown are CD31⁻CD45⁻CD24⁺. C, quantification of each subpopulation in Jarid1b^+/+/Jarid1b^+/− and Jarid1b^−/− MECs. For the combined Jarid1b^+/+ and Jarid1b^+/− group, n = 3 for Jarid1b^+/+ and n = 2 for Jarid1b^+/−; for Jarid1b^−/−, n = 5. Error bars represent S.E.