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JARID1B directly regulates mammary development genes through both GATA3-dependent and -independent mechanisms. A, B, and C, ChIP analysis of H3K4me3 (A), JARID1B (B), and GATA3 (C) enrichment at the promoter regions of a panel of previously described mammary development genes. PCR primers were designed to identify genomic regions ranging from −2 to 0.3 kb relative to the TSS of each gene. Exact primer locations are noted in supplemental Table 1. Error bars represent S.E.
