FIGURE 1.

Loss of SRC-2 disrupts cardiac transcription factor target gene expression. A, immunohistochemistry of heart sections taken from adult WT or SRC-2 CKO mice with anti-SRC-2 antibodies. B, qPCR analysis of mRNA expression of SRC-2 in hearts and liver RNA from adult WT or SRC-2 CKO mice. Individual gene expression is normalized to an 18S rRNA internal control. C, mRNA expression analysis via qPCR for cardiac transcription factors in RNA isolated from WT and SRC-2 CKO hearts as in A. D, gene set enrichment analysis (GSEA) transcription factor motif enrichment of genes altered in the heart from loss of SRC-2 (17) with a False Discovery Rate ≤ 0.15. Bold lanes highlight well characterized cardiac transcription factors. E, genes used in D were analyzed for MEF2, GATA-4, and Tbx5 binding by ChIP-Seq analysis in HL-1 cells (2). Statistical analysis was performed with Student's t test where * = p ≤ 0.05, ** = p ≤ 0.01, and *** = p ≤ 0.001.