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Proteasome-mediated degradation of MBP in mammalian cells is not affected by antizyme. HEK293 cells were transfected with cDNAs coding for ODC or rhMBP, in addition to cDNAs coding for the respective ubiquitin species, as indicated. cDNAs coding for antizyme (AZ) were co-transfected with ODC at different antizyme:protein ratios: 1:10 (A) and 1:100 (B and C). After 24 h, the cells were treated by cycloheximide (CHX), as indicated and subjected to Western blotting analysis.
