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Estimation of antibody avidity to gp120 BaL. Ab21 was diluted to 1 μm and exposed to 10 μm hematin. Heme-treated Ab21 was diluted 2 μg/ml in T-PBS. After incubation with Ab21, the microtitration plate was washed extensively with T-PBS and incubated for 10 min at 37 °C with increasing concentrations of urea as follows: 0, 0.5, and 1–8 m in PBS. After extensive washing of the plate with T-PBS, the immunoreactivity was developed as described above.
