FIGURE 6.

Temperature dependence of association of Ab21, F425 B4a1, and b12 with gp120. Arrhenius plots depict the temperature dependence of association rate constant of Ab21-heme (black squares), F425 B4a1 (white circles), and b12 (gray diamonds) obtained after interaction with different variants of gp120. The association rate constants were determined by global analysis of sensorograms generated after evaluation of binding kinetics of Ab21-heme, F425 B4a1, and b12 (as described under “Experimental Procedures”) at varying temperatures (10, 15, 20, 25, 30, and 35 °C). The slopes of the Arrhenius plots were determined by linear regression analyses. The temperature dependence of binding of b12 to 96ZM651 displayed complex behavior. The data were fitted by linear regression analyses in two alternative ranges, 10–25 and 25–35 °C.