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Effect of Tyr on CHO cells producing a recombinant monoclonal antibody. A, residual Tyr concentration. B, residual Phe concentration. C, specific productivity. D, titer. E, percentage of viability. F, integrated viable cell density (IVCD). Symbols: dashed line (□), cell culture without Tyr supplementation; solid line (■), cell culture with Tyr supplementation. Arrows indicate timing of tyrosine addition. Cells were grown in chemically defined media.
