FIGURE 2.
Nodal and Gdf1 can synergize independently of the Nodal prodomain and precursor cleavage motif. A, induction of CAGA-luc by Nmat, which lacks the prodomain, or Nmat·Gdf1(HA) in complete conditioned media. B, induction of CAGA-luc by complete media conditioned with Nr or Nr·Gdf1(His6). 600 ng/ml rNodal was analyzed for comparison. Top, full-length Nodal precursor detected with the M2 anti-FLAG antibody. C, induction of CAGA-luc by complete media conditioned by transient transfection of combinations of Nodal (N) and Gdf1(HA). mat, mature domain only; pro, prodomain only; r, resistant to proprotein convertase cleavage. In A, error bars represent S.D.