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. 2014 May 5;289(25):17854–17871. doi: 10.1074/jbc.M114.550301

FIGURE 6.

FIGURE 6.

Purified Nodal·Gdf1 complex has high specific activity in serum-free conditions and stimulates differentiation of hESCs into endoderm progenitors. A, induction of CAGA-luc by recombinant TGFβ (dotted line), Activin (gray line), Nodal (dashed line), and Nodal·Gdf1 (black line). B, induction of CAGA-luc by recombinant Nodal (50 ng/ml) or affinity-purified Nodal·Gdf1 (12.5 ng/ml) previously incubated in medium containing decreasing FBS concentrations. C, immunohistochemistry analysis of SOX17 and OCT4 in undifferentiated hESCs and cells differentiated with Wnt3a and basic FGF (Vehicle). D, CXCR4 and EpCam FACS profile of hESCs treated with 100 ng/ml Activin A, Nodal, Nodal·Gdf1, or vehicle. Asterisks indicate p values relative to the vehicle control. *, p ≤ 0.05; ***, p ≤ 0.001. E, RT-PCR analysis of selected markers following treatment of hESCs with the same factors. p values (above the graph) were calculated relative to the vehicle control. In A and B, error bars represent S.D. In E, error bars represent S.E.

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