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. 2014 May 5;289(25):17854–17871. doi: 10.1074/jbc.M114.550301

FIGURE 7.

FIGURE 7.

Gdf1 is needed for efficient signaling via Cripto or Cryptic but does not enhance binding of Nodal to soluble receptor Fc fusion proteins. A, induction of CAGA-luc by recombinant Nodal (gray) or purified Nodal·Gdf1 (black) in reporter cells expressing Cripto (top) or Cryptic (bottom). Western blot (WB) analysis of Fc-receptor fusion constructs and associated Nodal proteins following pulldown (PD) of Cripto-Fc (B) or Cryptic-Fc (C) fusion proteins using Gdf1(HA) is shown. D, induction of CAGA-luc by recombinant Nodal or Nodal·Gdf1(His6) following treatment of the reporter cells with siRNAs against ACVR2B or BMPR2. Western blot analysis of Fc-receptor fusion constructs and associated Nodal proteins following pulldown of ACVR1B-Fc (E) or Acvr2a-Fc and Acvr2b-Fc (F) fusion proteins using Gdf1(HA) is shown. In A and D, error bars represent S.D.

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