FIGURE 8.
Differential inhibition of Nodal and Nodal·Gdf1 by soluble type II receptors. A, induction of CAGA-luc by Nodal or Nodal·Gdf1(HA) conditioned medium preincubated with increasing concentrations of the indicated type II receptor extracellular domain fused to Fc. B, induction of CAGA-luc by Activin conditioned medium that was preincubated with increasing concentrations of the indicated Fc-receptor fusion protein. C, Western blot (WB) analysis of Fc-receptor fusion constructs and associated Nodal proteins following pulldown of the fusion proteins. D, induction of CAGA-luc by Nodal or Nodal·Gdf1(HA) conditioned medium that was preincubated with increasing concentrations of the indicated type II receptor extracellular domain fused to Fc. In A, B, and D, error bars represent S.D. LIF, leukemia inhibitory factor.