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. 2014 May 5;289(25):17854–17871. doi: 10.1074/jbc.M114.550301

TABLE 2.

Identification of Nodal and Gdf1 from purified material

Listed are normalized spectral counts of Nodal and Gdf1 identified after affinity purification of Nodal·Gdf1(His6) and Nodal·Gdf1(HA) using a nickel column. Fold, -fold change between Gdf1(HA) and Gdf1(His6). All other proteins did not show a -fold change higher than 1 or more than 3 spectral counts. Mature and pro-Nodal are found in bands 1 and 3, respectively. Mature and pro-Gdf1 are found in bands 2 and 3, respectively.

Protein description Species UniProt no. Molecular weight -Fold Nodal·Gdf1(His6)
Nodal·Gdf1(HA)
Band 1 Band 2 Band 3 Band 1 Band 2 Band 3
kDa
Embryonic growth/differentiation factor 1 M. musculus P20863 39 7.6 40 83 12 5 13 0
Nodal M. musculus P43021 40 10 31 9 49 5 2 2
HHS Vulnerability Disclosure