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. 2014 May 14;289(25):17895–17908. doi: 10.1074/jbc.M113.536565

FIGURE 4.

FIGURE 4.

Altered fibrillar amyloid deposition in bigenic Tg-SwDI/Tg-5xFAD mouse brains. Shown are representative images of 3- to 9-month-old transgenic mouse brain sections stained for fibrillar amyloid using thioflavin S (green) and immunolabeled for cerebral blood vessels using an antibody to collagen IV (red), as described under “Experimental Procedures.” A–C, Tg-SwDI mice. D–F, Tg-5xFAD mice. G–I, bigenic Tg-SwDI/Tg-5xFAD mice. Scale bars = 50 μm. The amount of cerebral microvascular amyloid (J) and the size distribution of all fibrillar amyloid deposits in 3- (K), 6- (L), and 9-month-old (M) Tg-SwDI mice (black bars), Tg-5xFAD mice (blue bars), and bigenic Tg-SwDI/Tg-5xFAD mice (red bars) was determined in the subiculum region using stereological principles, as described under “Experimental Procedures.” Data are the mean ± S.D. of eight mice per group. a, p < 0.05; b, p < 0.01; c, p < 0.001; d, p < 0.0001; ns, not significant.