FIGURE 8.

Non-mutated parenchymal amyloid deposits in Tg-5xFAD mice promote the accumulation of injected biotin-labeled Dutch/Iowa CAA mutant Aβ. Biotin-labeled Dutch/Iowa CAA mutant Aβ40 (A–D) and biotin (E–H) were injected into the hippocampal region of 6-month-old Tg-5xFAD mice. Brain sections were prepared, fibrillar amyloid was visualized by staining with thioflavin S (green), and biotin-labeled Dutch/Iowa CAA mutant Aβ40 or biotin alone was detected using Texas Red-labeled streptavidin (red). A–C and E–G, scale bars = 50 μm. D and H, scale bars = 12.5 μm.