FIGURE 7.

ASN12 is taken up by neurons in the CNS of the Line41 APPtg mouse. Line41 APPtg and non-tg mice received intraperitoneal injections of ASN12 or SN5 (1 mg/kg) or saline twice weekly for 3 weeks. A, brain sections were stained for human neprilysin (red), the neuronal marker NeuN (green), and counterstained for nuclei (DAPI, blue). B, quantitation of % co-localization of neprilysin and NeuN staining. C, brain sections stained for neprilysin (red), the astrocytic marker GFAP (green), and counterstained for nuclei (DAPI, blue). D, quantitation of % co-localization of neprilysin and GFAP staining. * indicates ANOVA p < 0.05 compared with mice that received SN5. Arrows highlight cells staining for neprilysin. Scale bars = 7 μm. n = 5 for each group.