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. 2014 May 14;289(25):17932–17940. doi: 10.1074/jbc.M114.564534

FIGURE 2.

FIGURE 2.

A–F, thermograms and binding isotherms with theoretical fits for the binding of (GlcN)2–7 to OfChtI: (GlcN)2 (A), (GlcN)3 (B), (GlcN)4 (C), (GlcN)5 (D), (GlcN)6 (E), and (GlcN)7 (F). G, free energy changes for (GlcN)2–7 binding to OfChtI relative to the number of GlcN units in the saccharide chain. (GlcN)2 and (GlcN)3 bind to OfChtI at two sites. For (GlcN)2, S1 is the strong binding site with a low Kd value, and S2 is the weak binding site with a high Kd value. For (GlcN)3, S1′ is the strong binding site with a low Kd value, and S2′ is the weak binding site with a high Kd value.