TABLE 4.

Thermodynamic parameters of (GlcN)₅ binding to OfChtI and the mutant E148Q derived from ITC at 30 °C (pH 8.0)

Protein	Ligand	n	Kd	ΔG	ΔH	−TΔS
			μm	kcal mol−1	kcal mol−1	kcal mol−1
OfChtI	(GlcN)5	1.02 ± 0.03	3.9 ± 0.5	−7.48	−11.63 ± 0.34	4.15
E148Q	(GlcN)5	0.91 ± 0.04	73.0 ± 13.2	−5.73	−2.71 ± 0.27	−3.02