Fig. 8.

Effects of coexpression conditions on plasma membrane targeting of AQP2-wt. Oocytes were injected either with pure HA-AQP2-wt (0.5 ng) or with the same mRNA plus untagged AQP2-D150E (10 ng) or AQP2-G196D (10 ng) and incubated for 2 days prior to assay. Oocytes were treated by preparation of total membrane or purified plasma membrane fractions as described in methods. An equivalent of 1 oocyte per condition was loaded for total membranes (A) and plasma membrane lanes employed purified fractions from 40 oocytes (B); blots were probed using anti-HA antibody to specifically visualize AQP2-wt.