Figure 1. Putative pathway for scutellarin and chlorogenic acids biosynthesis in E. breviscapus.

The major products previously found in E. breviscapus are surrounded by shaded boxes. Enzymes found in this study are boxed; new putative enzyme (flavone 6-hydroase, F6H) was surrounded by dashed line box. Enzymes involved in the pathways are: 4CL, 4-coumarate: CoA ligase; C3H, p-coumarate 3-hydroxylase; C4H, cinnamate 4-hydroxylase; CHI, chalcone isomerase; CHS, chalcone synthase, DFR, dihydroflavonol 4-reductase; F3′5′H flavonoid 3′5′ hydroxylase; F3′H, flavonoid 3′ hydroxylase; F3H, flavanone 3-hydroxylase  =  flavonol synthase (FLS); FSII, flavone synthase II; HCGQT, hydroxycinnamoyl D-glucose: quinate hydroxycinnamoyl transferase; HCT, hydroxycinnamoyl CoA shikimate/quinate hydroxycinnamoyl transferase; HQT, hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase; LDOX, leucoanthocyanidin dioxygenase  =  anthocyanidin synthase (ANS); OMT, O-methyltransferase; PAL, phenylalanine ammonia lyase; RT, rhamnosyl transferase; UFGT, UDPG-flavonoid glucosyl transferase; UGCT, UDP glucose: cinnamate glucosyl transferase; F7GAT, flavonoid 7-O-glucuronosyltransferase.