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. 2014 Jun 23;9(6):e100619. doi: 10.1371/journal.pone.0100619

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© 2014 Forberg et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Blood was taken from influenza A virus infected (n = 12) and control pigs (n = 9) on day 0−3 pi in a second experiment. (A) Isolated PBMCs were analysed by flow cytometry and live CD3⁻ lymphocytes were gated as NKp46⁻ or NKp46⁺ NK cells according to CD8α and NKp46 expression. Plots are taken from a representative control animal. Absolute numbers of (B) NKp46⁺ NK cells in PBMC were analysed by flow cytometry in control (left) and infected animals (right). (C) Results for the NKp46⁺ cells in the two groups were compared on each sampling day. (D) NKp46⁻ NK cells in PBMC in control (left) and infected animals (right). (E) NKp46⁻ NK cells were compared for the two groups. Each line in (B) and (D) represents one animal. **p≤0.01.