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Recombinant human CKB (0.4 mg/mL) was aged in vitro at pH 7.5 and 37°C for 16 days. (A) At four-day intervals, samples were taken to assay CKB activity and isoAsp levels. Assays were performed in triplicate with standard deviations indicated by error bars. (B) SDS-PAGE of in vitro aged human recombinant CKB showing lack of significant degradation or aggregation.
