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. 2014 Jun 23;9(6):e100622. doi: 10.1371/journal.pone.0100622

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© 2014 Dimitrijevic et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Aged recombinant human CKB was subjected to in vitro repair as described under “Materials and Methods” in the presence of PIMT and unlabeled AdoMet. CKB activity (right axis) before aging, after aging and after repair was determined and isoAsp content (left axis) was measured by a methanol diffusion assay. Each assay was performed in triplicate with error bars representing standard deviation.