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. 2014 Jun 23;9(6):e100657. doi: 10.1371/journal.pone.0100657

Figure 3. Overexpression of miR-146b promotes myoblast differentiation.

Figure 3

(A) C2C12 myoblasts were transfected with 50 nM miRIDIAN miR-146b mimic for 1 day and then induced to differentiate for 3 days. A C elegans miRNA (cel-67) mimic was used as negative control. The differentiated cells were fixed and immunostained for MHC (green) and DAPI (red). (B) Fusion indexes for the cells described in A were quantified. In addition, cells transfected with 50 nM native miR-146b duplex, with an siRNA against EGFP (siEGFP) as control, were differentiated and quantified for fusion index. (C) Cells described in A were lysed and subjected to Western blot analysis. In A and C, representative results of at least three independent experiments are shown. Data in B is the mean ± SD from three independent experiments. Paired t test was performed to compare the data. *P<0.05.