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. 2014 Jun 23;9(6):e100657. doi: 10.1371/journal.pone.0100657

Figure 4. Overexpression of miR-146b suppresses expression of Smad4, Hmga2 and Notch1 in myoblasts.

Figure 4

(A) C2C12 myoblasts were transfected with 50 nM miRIDIAN miR-146b mimic or cel-miR-67 mimic as control. After 24 hours, cells were lysed for RNA isolation, followed by qRT-PCR to measure mRNA levels for the genes shown. Relative mRNA levels are shown with that of cel-miR-67 as 1. Data shown are the mean ± SD from three independent experiments. One-sample t test was performed. **P<0.01. (B) Cells as described in A were lysed after 48 hours of transfection and subjected to Western blotting analysis. Representative results of three independent experiments are shown.