Figure 4. Normal Sry expression, but disrupted Sox9 expression, in XY Fgfr2^hob/hob embryonic gonads.

A) Sry WMISH at 11.5 dpc (16 ts) showing expression in XY wild-type (left) and XY Fgfr2^hob/hob (right) gonads. B, C) anti-SRY immunostaining at 18 ts in wild-type (B) and XY Fgfr2^hob/hob (C) gonads. D) Sox9 WMISH at 18 ts with tissue samples as described in (A). E, F) anti-SOX9 immunostaining at 18 ts in XY wild-type (E) and XY Fgfr2^hob/hob (F) gonads. G) Sox9 WMISH at 23 ts in XY wild-type (left) and XY Fgfr2^hob/hob (right) gonads. H, I) anti-SOX9 immunostaining at 23 ts in XY wild-type (H) and XY Fgfr2^hob/hob (I) gonads. J) Sox9 WMISH at 12.5 dpc in XY wild-type (left) and XY Fgfr2^hob/hob (right) gonads. K, L) anti-SOX9 immunostaining at 13.0 dpc in wild-type (K) and XY Fgfr2^hob/hob (L) gonads.