Skip to main content
. 2014 Jun 23;9(6):e100819. doi: 10.1371/journal.pone.0100819

Figure 2. Knockdown of Beclin 1, UVRAG, and ATG5 increase radiation-induced 53BP1, but not RAD51, nuclear foci.

Figure 2

A, B, Immunofluorescence staining for 53BP1 (A) or RAD51 (B) was performed in HT-29 cells with knockdown of Beclin 1, UVRAG or ATG5 and exposed to radiation (2 Gy) at the indicated times. The percentage of cells with >10 nuclear foci expressing either 53BP1 or RAD51 was calculated and plotted as shown. 53BP1 and RAD51 are markers of nonhomologous end joining and homologous recombination, respectively. DAPI was utilized to counterstain the nucleus. Data are presented as mean ± standard deviation for experiments performed in triplicate. Statistical significance was determined by a two-sided Student’s t test and defined as *P<0.05.