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. 2014 Jun 23;9(6):e100819. doi: 10.1371/journal.pone.0100819

Figure 4. Suppression of Beclin 1, UVRAG or both sensitize cells to DNA damage and apoptosis.

Figure 4

A, B, HT-29 and DLD1 cells were transfected with Beclin 1 (A) or UVRAG (B) vs control siRNA. Cells were irradiated and the expression of the indicated proteins was analyzed at 24 h post-radiation by immunoblotting. C, Time course of the effect of radiation on pATM expression in cells with UVRAG vs control siRNA. D, Effect of radiation on DNA damage and apoptosis markers in cells with dual knockdown of Beclin 1 and UVRAG vs UVRAG siRNA alone (24 h). Densitometry was performed and normalized against tubulin.