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Sildenafil reduces sarcolemmal and vascular permeability of mdx diaphragm muscle tissue. (A) Whole mounts of diaphragm respiratory muscles showing extensive accumulation of Evans Blue dye (EBD) in mdx diaphragms which was reduced by sildenafil treatment. (B) Quantification of EBD uptake. Sildenafil significantly reduces EBD uptake by the mdx diaphragm. EBD uptake studies were performed in four mice for each group and representative images are shown.
